Western Blot:Article Title: Acetylation of lysine 49 on Ctnnb1 drives naïve pluripotency in murine stem cells by modulating Nanog function
Article Snippet: .. Application Antibody (Primary antibody) Company Cat No (Clone No) Dilution Condition WB Mouse monoclonal anti-Oct3/4 Antibody Santacruz sc-5279 (C-10) 1/1000 in Immuno-enhancer 4°C, Overnight WB Rabbit monoclonal anti-Nanog Antibody CST #8822 (D2A3) 1/1000 in Immuno-enhancer 4°C, Overnight WB Goat polyclonal anti-Sox2 Antibody Santacruz sc-17320 (Y-17) 1/1000 in Immuno-enhancer 4°C, Overnight WB Goat polyclonal anti-LKLF(Klf4) Antibody Santacruz sc-18690 (N-13) 1/1000 in Immuno-enhancer 4°C, Overnight WB Rabbit polyclonal anti-TCF3 Antibody Santacruz sc-8635 (M-20) 1/1000 in Immuno-enhancer 4°C, Overnight WB Rabbit monoclonal anti-HDAC2 Antibody CST #2540 1/1000 in Immuno-enhancer 4°C, Overnight WB Rabbit monoclonal anti-CBP Antibody CST #7389 1/2500 in Immuno-enhancer 4°C, Overnight WB Rabbit monoclonal anti-p300 Antibody CST #86377 1/2500 in Immuno-enhancer 4°C, Overnight WB/IF Mouse polyclonal anti- βcatenin(14/βcatenin) Antibody BD Bioscience s 610153 WB:1/2000 in Immunoenhancer/ IF:1/200 in 10% Block-Ace/TBS 4°C, Overnight WB/IF Rabbit monoclonal anti-Acetyl-β- catenin(Lys49) antibody CST #9030 WB:1/1000 in Immuno- enhancer/ IF:1/200 in 10% Block-Ace/TBS 4°C, Overnight WB/IF Rabbit polyclonal anti-Catenin-beta (Acetyl Lys49) antibody BT LAB BT-AP01077 WB:1/2000 in Immuno- enhancer/ IF:1/1000 in 10% Block-Ace/TBS 4°C, Overnight WB Mouse monoclonal anti-Gapdh antibody FUJIFILM 014-25524 (5A12) 1 / 2,000 in Immunoenhancer 4°C, Overnight WB Rabbit monoclonal anti-TBP antibody CST #8515 1 / 1,000 in Immunoenhancer 4°C, Overnight WB Rabbit polyclonal anti-EP300 antibody CST #57625 (E8S2V) 1 / 2,500 in Immunoenhancer 4°C, Overnight ELISA Mouse monoclonal anti-Ep300 antibody Santacruz sc-48343 (F-4) 1/500 in 50% Block-Ace/TBS 37°C, 1h ELISA Rabbit polyclonal anti-Phospho-Ep300Ser89 Antibody St John's Laboratory STJ91120 1/2500 in in 50% BlockAce/TBS 37°C, 1h Wb Goat Anti-mouse IgG, HRP-linked Anbitody CST #7073 1/2000 in Immuno-enhancer 4°C, Overnight Wb Goat Anti-rabbit IgG, HRP-linked Anbitody CST #7074 1/2000 in Immuno-enhancer 4°C, Overnight IF Donkey Anti- IgG(H+L), Rabbit CFTM555 BTI 200038-1MG 1/1000 in 10% Block-Ace/TBS RT, 1h IF Donkey Anti- IgG(H+L), Mouse CFTM488 BTI 200038-1MG 1/1000 in 10% Block-Ace/TBS RT, 1h ..
Blocking Assay:Article Title: Acetylation of lysine 49 on Ctnnb1 drives naïve pluripotency in murine stem cells by modulating Nanog function
Article Snippet: .. Application Antibody (Primary antibody) Company Cat No (Clone No) Dilution Condition WB Mouse monoclonal anti-Oct3/4 Antibody Santacruz sc-5279 (C-10) 1/1000 in Immuno-enhancer 4°C, Overnight WB Rabbit monoclonal anti-Nanog Antibody CST #8822 (D2A3) 1/1000 in Immuno-enhancer 4°C, Overnight WB Goat polyclonal anti-Sox2 Antibody Santacruz sc-17320 (Y-17) 1/1000 in Immuno-enhancer 4°C, Overnight WB Goat polyclonal anti-LKLF(Klf4) Antibody Santacruz sc-18690 (N-13) 1/1000 in Immuno-enhancer 4°C, Overnight WB Rabbit polyclonal anti-TCF3 Antibody Santacruz sc-8635 (M-20) 1/1000 in Immuno-enhancer 4°C, Overnight WB Rabbit monoclonal anti-HDAC2 Antibody CST #2540 1/1000 in Immuno-enhancer 4°C, Overnight WB Rabbit monoclonal anti-CBP Antibody CST #7389 1/2500 in Immuno-enhancer 4°C, Overnight WB Rabbit monoclonal anti-p300 Antibody CST #86377 1/2500 in Immuno-enhancer 4°C, Overnight WB/IF Mouse polyclonal anti- βcatenin(14/βcatenin) Antibody BD Bioscience s 610153 WB:1/2000 in Immunoenhancer/ IF:1/200 in 10% Block-Ace/TBS 4°C, Overnight WB/IF Rabbit monoclonal anti-Acetyl-β- catenin(Lys49) antibody CST #9030 WB:1/1000 in Immuno- enhancer/ IF:1/200 in 10% Block-Ace/TBS 4°C, Overnight WB/IF Rabbit polyclonal anti-Catenin-beta (Acetyl Lys49) antibody BT LAB BT-AP01077 WB:1/2000 in Immuno- enhancer/ IF:1/1000 in 10% Block-Ace/TBS 4°C, Overnight WB Mouse monoclonal anti-Gapdh antibody FUJIFILM 014-25524 (5A12) 1 / 2,000 in Immunoenhancer 4°C, Overnight WB Rabbit monoclonal anti-TBP antibody CST #8515 1 / 1,000 in Immunoenhancer 4°C, Overnight WB Rabbit polyclonal anti-EP300 antibody CST #57625 (E8S2V) 1 / 2,500 in Immunoenhancer 4°C, Overnight ELISA Mouse monoclonal anti-Ep300 antibody Santacruz sc-48343 (F-4) 1/500 in 50% Block-Ace/TBS 37°C, 1h ELISA Rabbit polyclonal anti-Phospho-Ep300Ser89 Antibody St John's Laboratory STJ91120 1/2500 in in 50% BlockAce/TBS 37°C, 1h Wb Goat Anti-mouse IgG, HRP-linked Anbitody CST #7073 1/2000 in Immuno-enhancer 4°C, Overnight Wb Goat Anti-rabbit IgG, HRP-linked Anbitody CST #7074 1/2000 in Immuno-enhancer 4°C, Overnight IF Donkey Anti- IgG(H+L), Rabbit CFTM555 BTI 200038-1MG 1/1000 in 10% Block-Ace/TBS RT, 1h IF Donkey Anti- IgG(H+L), Mouse CFTM488 BTI 200038-1MG 1/1000 in 10% Block-Ace/TBS RT, 1h ..
Enzyme-linked Immunosorbent Assay:Article Title: Acetylation of lysine 49 on Ctnnb1 drives naïve pluripotency in murine stem cells by modulating Nanog function
Article Snippet: .. Application Antibody (Primary antibody) Company Cat No (Clone No) Dilution Condition WB Mouse monoclonal anti-Oct3/4 Antibody Santacruz sc-5279 (C-10) 1/1000 in Immuno-enhancer 4°C, Overnight WB Rabbit monoclonal anti-Nanog Antibody CST #8822 (D2A3) 1/1000 in Immuno-enhancer 4°C, Overnight WB Goat polyclonal anti-Sox2 Antibody Santacruz sc-17320 (Y-17) 1/1000 in Immuno-enhancer 4°C, Overnight WB Goat polyclonal anti-LKLF(Klf4) Antibody Santacruz sc-18690 (N-13) 1/1000 in Immuno-enhancer 4°C, Overnight WB Rabbit polyclonal anti-TCF3 Antibody Santacruz sc-8635 (M-20) 1/1000 in Immuno-enhancer 4°C, Overnight WB Rabbit monoclonal anti-HDAC2 Antibody CST #2540 1/1000 in Immuno-enhancer 4°C, Overnight WB Rabbit monoclonal anti-CBP Antibody CST #7389 1/2500 in Immuno-enhancer 4°C, Overnight WB Rabbit monoclonal anti-p300 Antibody CST #86377 1/2500 in Immuno-enhancer 4°C, Overnight WB/IF Mouse polyclonal anti- βcatenin(14/βcatenin) Antibody BD Bioscience s 610153 WB:1/2000 in Immunoenhancer/ IF:1/200 in 10% Block-Ace/TBS 4°C, Overnight WB/IF Rabbit monoclonal anti-Acetyl-β- catenin(Lys49) antibody CST #9030 WB:1/1000 in Immuno- enhancer/ IF:1/200 in 10% Block-Ace/TBS 4°C, Overnight WB/IF Rabbit polyclonal anti-Catenin-beta (Acetyl Lys49) antibody BT LAB BT-AP01077 WB:1/2000 in Immuno- enhancer/ IF:1/1000 in 10% Block-Ace/TBS 4°C, Overnight WB Mouse monoclonal anti-Gapdh antibody FUJIFILM 014-25524 (5A12) 1 / 2,000 in Immunoenhancer 4°C, Overnight WB Rabbit monoclonal anti-TBP antibody CST #8515 1 / 1,000 in Immunoenhancer 4°C, Overnight WB Rabbit polyclonal anti-EP300 antibody CST #57625 (E8S2V) 1 / 2,500 in Immunoenhancer 4°C, Overnight ELISA Mouse monoclonal anti-Ep300 antibody Santacruz sc-48343 (F-4) 1/500 in 50% Block-Ace/TBS 37°C, 1h ELISA Rabbit polyclonal anti-Phospho-Ep300Ser89 Antibody St John's Laboratory STJ91120 1/2500 in in 50% BlockAce/TBS 37°C, 1h Wb Goat Anti-mouse IgG, HRP-linked Anbitody CST #7073 1/2000 in Immuno-enhancer 4°C, Overnight Wb Goat Anti-rabbit IgG, HRP-linked Anbitody CST #7074 1/2000 in Immuno-enhancer 4°C, Overnight IF Donkey Anti- IgG(H+L), Rabbit CFTM555 BTI 200038-1MG 1/1000 in 10% Block-Ace/TBS RT, 1h IF Donkey Anti- IgG(H+L), Mouse CFTM488 BTI 200038-1MG 1/1000 in 10% Block-Ace/TBS RT, 1h ..
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